RNA-Seq of Pinus sylvestris ramets - SRA

SRX24662877: RNA-Seq of Pinus sylvestris ramets
1 ION_TORRENT (Ion Torrent PGM) run: 1.3M spots, 234M bases, 183.2Mb downloads

Design: RNA samples for transcriptome sequencing were enriched for polyadenylated mRNAs from the total RNA samples using the Dynabeads mRNA DIRECT Micro Kit (Life Technologies). The quantity after transcript enrichment of RNA from one of the treated samples (Sm8) was not sufficient for library construction; therefore, five transcriptome libraries were produced using the Ion Total RNA-Seq Kit v2 (Life Technologies) and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute of Bioeconomy Research (NIBIO) according to the manufacturers instructions and as reported previously (Knberga-Silia et al., 2017). The sequences were analysed on the Ion Torrent Server version 4.0.2.

Submitted by: Latvian State Forest Research Institute "Silava"

Study: Differential miRNA expression in Scots pine (Pinus sylvestris) needles in response to methyl jasmonate treatment

PRJNA1115068 • SRP509381 • All experiments • All runs

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The aim of this study was to investigate the effect of methyl jasmonate MeJA treatment on miRNA and gene expression in Scots pine needles and to compare expression levels of miRNAs and their target genes. Potential precursor sequences and target genes of the identified miRNAs were determined, to understand the type of processes regulated by both conserved and novel miRNAs under stress conditions. In this study, a combined strategy-high throughput sequencing and computational prediction was utilized to investigate the effect of MeJA on Scots pine miRNAs and the transcriptome. Differentially expressed miRNAs and target genes in MeJA treated Scots pine needles are identified.

Sample: Techical repeat of sample Sm4 whole transcriptome seq from Pinus sylvestris, needles of 1 year old ramet

SAMN41506351 • SRS21398236 • All experiments • All runs

Organism: Pinus sylvestris

Library:

Name: wt_Sm4_repeated

Instrument: Ion Torrent PGM

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 1.3M spots, 234M bases, 183.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR29141112	1,261,026	234M	183.2Mb	2024-05-23

ID:: 32981785

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